Review of recently reported Ricin detection techniques focusing on combined immunoassay detection with abrin and saxitoxin in human plasma

Seungmin Kang; Seonhee Kim; David G. Churchill; Kang Ku; Yoonjeong Jang

doi:10.37944/jams.v6i2.191

Authors

Seungmin Kang CBRN Defense Research Institute https://orcid.org/0000-0002-0690-7039
Seonhee Kim 7th Infantry division, CBRN Battalion
David G. Churchill Korea Advanced Institute of Science and Technology (KAIST)
Kang Ku Chemical Analysis Department, CBRN Defense Research Institute
Yoonjeong Jang CBRN Defense Research Institute, Biological Analysis Department

DOI:

https://doi.org/10.37944/jams.v6i2.191

Keywords:

biotoxin, ricin, abrin, prohibition of chemical weapons, immunoassay detection platforms

Abstract

Increasing non-traditional threats from biological or chemical weapons, the Organisation for the Prohibition of Chemical Weapons (OPCW) have tried to perform the preliminary analysis of biotoxin sample to standardize analysis methods and strengthen analytical capabilities among OPCW member countries. With the changes of new analysis, ROK CBRN Defense Research Institute (CDRI) established enzyme-linked immunosorbent assay (ELISA) and cytotoxicity analysis methods for ricin, abrin, and saxitoxin through the OPCW exercise on Biotoxin sample analysis. Thus, this study aims to established analytical methods (ELISA and cytotoxicity analysis) for the biological toxins called ricin, abrin and saxitoxin according to recent OPCW Biotoxin detection exercise. In particular, to refine practical and effective methods of biological analysis, we reviewed recent research on scientific analysis of ricin as a potential bioterror weapon, letter with ricin sent in White House, and suggested future agendas for preparedness testing.

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Author Biographies

Seungmin Kang, CBRN Defense Research Institute

(First Author) CBRN Defense Research Institute, Biological Analysis Department, Microbiology Research Officer, [email protected], https://orcid.org/0000-0002-0690-7039.

Seonhee Kim, 7th Infantry division, CBRN Battalion

(Co-Author) 7th Infantry division, CBRN Battalion, CBRN Battalion Commander, [email protected], https://orcid.org/0000-0002-2002-0135.

David G. Churchill, Korea Advanced Institute of Science and Technology (KAIST)

(Co-Author) Korea Advanced Institute of Science and Technology (KAIST), Department of Chemistry, Professor, [email protected], https://orcid.org/0000-0002-2520-4638.

Kang Ku, Chemical Analysis Department, CBRN Defense Research Institute

(Co-Author) CBRN Defense Research Institute, Chemical Analysis Department, Head of Chemical Analysis Department, [email protected], https://orcid.org/0000-0001-8739-218X.

Yoonjeong Jang, CBRN Defense Research Institute, Biological Analysis Department

(Corresponding Author) CBRN Defense Research Institute, Biological Analysis Department, Head of Biological Analysis Department, [email protected], https://orcid.org/0000-0002-5625-2528.

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